Posters to Precede Presentation: April 22, 2021 6:00 PM CST
Posters by: Edward G. Franklin, PhD, Technical Director of Chromatography Applications at Regis Technologies, Inc.
HPLC-UV Method Development for Baseline Resolution of 17 Cannabinoids
With ongoing discussions regarding the legalization of marijuana along with great interest in the potential medical benefits of hemp-derived products, there is increasing demand upon the cannabis testing industry for analytical determination of cannabinoid content. Current regulations concerning potency vary by jurisdiction, but usually require testing for the active forms of THC and CBD. In addition to those, California requires testing for the acid forms, THCA and CBDA, along with CBG and CBN. As regulations evolve, and as research interests in minor cannabinoids expand, it is important to have robust analytical methods in place that are capable of meeting needs. Herein, the baseline resolution of 17 cannabinoids by high performance liquid chromatography (HPLC) with UV detection is described. Chromatographic method development was performed with particular attention to speed of analysis and means of affecting selectivity for the improved resolution of critical pairs.
Sample Loadability on Coated and Immobilized Polysaccharide-Based CSPs
Chiral stationary phases (CSPs) prepared by coating phenylcarbamate derivatives of amylose and cellulose on supporting silica gels have proven invaluable for the separation and purification of enantiomers in both high-performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC). One notable limitation of coated polysaccharide phases is the restricted use of certain organic mobile phases, such as acetone, chloroform, ethyl acetate, tetrahydrofuran (THF), and toluene. These solvents must be avoided as they can swell or dissolve the polysaccharide and destroy columns packed with coated CSPs. Immobilized versions of the same chiral selectors have expanded the capabilities of these phases to allow for the use of such solvents in analysis and sample purification. For some applications, however, practitioners have observed important differences in selectivity and sample loading capacities between the coated and immobilized versions. Herein, three examples of chiral separations performed using HPLC and SFC are discussed with respect to how these differences may be manifested and overcome or exploited to advantage.
Edward G. Franklin, PhD, Technical Director of Chromatography Applications at Regis Technologies, Inc. Dr. Edward G. Franklin earned a B.S. in Biochemistry in 2006 and M.S. in Forensic Science in 2007 from Duquesne University in Pittsburgh, PA. In 2012, he received a PhD in Analytical Chemistry from The University of North Carolina at Chapel Hill under the direction of Dr. James Jorgenson. In over 10 years of HPLC-related experience, he has conducted research ranging from fundamental investigations of the kinetic performance of packed-bed HPLC columns, instrument and column development for ultra-high pressure separations of complex samples, and column stationary phase development for application-specific methods. In his current position as the Technical Director of Chromatography Applications at Regis Technologies, Inc., he develops new products and methods for chromatography solutions related to chiral and achiral separations using reversed-phase, normal phase, and supercritical fluid approaches.
“I now have a better understanding of how HPLC works and why column choice is so important.”
“It is a fantastic course for learning the theory of LC and method development”
“The instructor, Merlin Bicking, is very helpful. It was great to see how wiling he is to answer questions and all the variety of methods he worked on.”
“I am just beginning my position from out of school. I found the course very insightful in terms of preparation for my future career”
“It is very useful for me because having hands on experience, which helped me to understand more of HPLC.”
“Great class. Would easily take again and recommend Dr. Merlin anytime. Would love to take his other courses. If he had a book would quickly buy for reference.”
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Last Updated: April 12, 2021 by CCDG
Posters to Precede Presentation: April 22, 2021 6:00 PM CST
Posters by: Edward G. Franklin, PhD, Technical Director of Chromatography Applications at Regis Technologies, Inc.
HPLC-UV Method Development for Baseline Resolution of 17 Cannabinoids
With ongoing discussions regarding the legalization of marijuana along with great interest in the potential medical benefits of hemp-derived products, there is increasing demand upon the cannabis testing industry for analytical determination of cannabinoid content. Current regulations concerning potency vary by jurisdiction, but usually require testing for the active forms of THC and CBD. In addition to those, California requires testing for the acid forms, THCA and CBDA, along with CBG and CBN. As regulations evolve, and as research interests in minor cannabinoids expand, it is important to have robust analytical methods in place that are capable of meeting needs. Herein, the baseline resolution of 17 cannabinoids by high performance liquid chromatography (HPLC) with UV detection is described. Chromatographic method development was performed with particular attention to speed of analysis and means of affecting selectivity for the improved resolution of critical pairs.
Click here to view poster.
Sample Loadability on Coated and Immobilized Polysaccharide-Based CSPs
Chiral stationary phases (CSPs) prepared by coating phenylcarbamate derivatives of amylose and cellulose on supporting silica gels have proven invaluable for the separation and purification of enantiomers in both high-performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC). One notable limitation of coated polysaccharide phases is the restricted use of certain organic mobile phases, such as acetone, chloroform, ethyl acetate, tetrahydrofuran (THF), and toluene. These solvents must be avoided as they can swell or dissolve the polysaccharide and destroy columns packed with coated CSPs. Immobilized versions of the same chiral selectors have expanded the capabilities of these phases to allow for the use of such solvents in analysis and sample purification. For some applications, however, practitioners have observed important differences in selectivity and sample loading capacities between the coated and immobilized versions. Herein, three examples of chiral separations performed using HPLC and SFC are discussed with respect to how these differences may be manifested and overcome or exploited to advantage.
Click here to view poster.
Edward G. Franklin, PhD, Technical Director of Chromatography Applications at Regis Technologies, Inc. Dr. Edward G. Franklin earned a B.S. in Biochemistry in 2006 and M.S. in Forensic Science in 2007 from Duquesne University in Pittsburgh, PA. In 2012, he received a PhD in Analytical Chemistry from The University of North Carolina at Chapel Hill under the direction of Dr. James Jorgenson. In over 10 years of HPLC-related experience, he has conducted research ranging from fundamental investigations of the kinetic performance of packed-bed HPLC columns, instrument and column development for ultra-high pressure separations of complex samples, and column stationary phase development for application-specific methods. In his current position as the Technical Director of Chromatography Applications at Regis Technologies, Inc., he develops new products and methods for chromatography solutions related to chiral and achiral separations using reversed-phase, normal phase, and supercritical fluid approaches.
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LC School Reviews
“I now have a better understanding of how HPLC works and why column choice is so important.”
“It is a fantastic course for learning the theory of LC and method development”
“The instructor, Merlin Bicking, is very helpful. It was great to see how wiling he is to answer questions and all the variety of methods he worked on.”
“I am just beginning my position from out of school. I found the course very insightful in terms of preparation for my future career”
“It is very useful for me because having hands on experience, which helped me to understand more of HPLC.”
“Great class. Would easily take again and recommend Dr. Merlin anytime. Would love to take his other courses. If he had a book would quickly buy for reference.”