Dr Paula Hong Principal Scientist, Waters Corporation
Methods Transfer Review: Strategies to Address the Impact of Instrument Design Characteristics
Transfer of established reversed phase methods across both HPLC and UHPLC chromatographic platforms requires careful consideration of the operating parameters and design of each instrument. To illustrate these affects, system characteristics, including dwell volume, gradient formation, extra-column dispersion and linearity will be reviewed and related to the differences in the resulting chromatography. Different instrument configurations will also be evaluated and discussed, including quaternary and binary pumps as well as flow through and off-line injection modes. Based on the effects of individual system characteristics, strategies for successful methods transfer will be described. In these examples, consideration is made to conduct method transfer in accordance with regulatory guidelines for allowable adjustments to compendial methods.
Bio:
Paula Hong is a Principal Scientist at Waters Corporation. She received her B.S. in Chemistry from Bucknell University (Lewisburg, PA) and her Ph.D. in Inorganic Chemistry from The University of Pennsylvania (Philadelphia,PA). She began her career as an applications chemist at Waters Corporation over 9 years ago in the area of bioseparations. At that time her work focused on UHPLC and sub-2um technology for the application of biomoelcules, including amino acid analysis, ion-exchange and size-exclusion chromatography. In her more recent work she has focused on the impact of instrumentation on numerous applications for both LC and SFC. She has authored or co-authored review articles as well as white papers, including one on both LC and SFC method development. Her more recent work has focused on method transfer across both Waters and other manufacturers systems.
LC School Reviews
“I now have a better understanding of how HPLC works and why column choice is so important.”
“It is a fantastic course for learning the theory of LC and method development”
“The instructor, Merlin Bicking, is very helpful. It was great to see how wiling he is to answer questions and all the variety of methods he worked on.”
“I am just beginning my position from out of school. I found the course very insightful in terms of preparation for my future career”
“It is very useful for me because having hands on experience, which helped me to understand more of HPLC.”
“Great class. Would easily take again and recommend Dr. Merlin anytime. Would love to take his other courses. If he had a book would quickly buy for reference.”
Dr. Paula Hong
Dr Paula Hong
Principal Scientist, Waters Corporation
Methods Transfer Review: Strategies to Address the Impact of Instrument Design Characteristics
Transfer of established reversed phase methods across both HPLC and UHPLC chromatographic platforms requires careful consideration of the operating parameters and design of each instrument. To illustrate these affects, system characteristics, including dwell volume, gradient formation, extra-column dispersion and linearity will be reviewed and related to the differences in the resulting chromatography. Different instrument configurations will also be evaluated and discussed, including quaternary and binary pumps as well as flow through and off-line injection modes. Based on the effects of individual system characteristics, strategies for successful methods transfer will be described. In these examples, consideration is made to conduct method transfer in accordance with regulatory guidelines for allowable adjustments to compendial methods.
Paula Hong is a Principal Scientist at Waters Corporation. She received her B.S. in Chemistry from Bucknell University (Lewisburg, PA) and her Ph.D. in Inorganic Chemistry from The University of Pennsylvania (Philadelphia,PA). She began her career as an applications chemist at Waters Corporation over 9 years ago in the area of bioseparations. At that time her work focused on UHPLC and sub-2um technology for the application of biomoelcules, including amino acid analysis, ion-exchange and size-exclusion chromatography. In her more recent work she has focused on the impact of instrumentation on numerous applications for both LC and SFC. She has authored or co-authored review articles as well as white papers, including one on both LC and SFC method development. Her more recent work has focused on method transfer across both Waters and other manufacturers systems.
LC School Reviews
“I now have a better understanding of how HPLC works and why column choice is so important.”
“It is a fantastic course for learning the theory of LC and method development”
“The instructor, Merlin Bicking, is very helpful. It was great to see how wiling he is to answer questions and all the variety of methods he worked on.”
“I am just beginning my position from out of school. I found the course very insightful in terms of preparation for my future career”
“It is very useful for me because having hands on experience, which helped me to understand more of HPLC.”
“Great class. Would easily take again and recommend Dr. Merlin anytime. Would love to take his other courses. If he had a book would quickly buy for reference.”