Dr. Paula Hong
Dr Paula Hong
Principal Scientist, Waters Corporation
Methods Transfer Review: Strategies to Address the Impact of Instrument Design Characteristics
Transfer of established reversed phase methods across both HPLC and UHPLC chromatographic platforms requires careful consideration of the operating parameters and design of each instrument. To illustrate these affects, system characteristics, including dwell volume, gradient formation, extra-column dispersion and linearity will be reviewed and related to the differences in the resulting chromatography. Different instrument configurations will also be evaluated and discussed, including quaternary and binary pumps as well as flow through and off-line injection modes. Based on the effects of individual system characteristics, strategies for successful methods transfer will be described. In these examples, consideration is made to conduct method transfer in accordance with regulatory guidelines for allowable adjustments to compendial methods.
Paula Hong is a Principal Scientist at Waters Corporation. She received her B.S. in Chemistry from Bucknell University (Lewisburg, PA) and her Ph.D. in Inorganic Chemistry from The University of Pennsylvania (Philadelphia,PA). She began her career as an applications chemist at Waters Corporation over 9 years ago in the area of bioseparations. At that time her work focused on UHPLC and sub-2um technology for the application of biomoelcules, including amino acid analysis, ion-exchange and size-exclusion chromatography. In her more recent work she has focused on the impact of instrumentation on numerous applications for both LC and SFC. She has authored or co-authored review articles as well as white papers, including one on both LC and SFC method development. Her more recent work has focused on method transfer across both Waters and other manufacturers systems.