Join us for a dinner and networking event Wednesday February 25, 2026 at the Westwood Tavern in Schaumburg, IL. The lecture will be presented by Elizabeth Sande. This presentation describes a rapid, efficient method for analyzing post-translational crosslinking in the extracellular matrix, which is crucial for understanding fibrotic diseases such as idiopathic pulmonary fibrosis (IPF). By streamlining LC-MS/MS sample analysis to just two minutes and introducing a tissue-based surrogate matrix for dihydroxylysinonorleucine (DHLNL), the process is less labor-intensive and provides more accurate quantification. Application of this method revealed elevated DHLNL levels in both IPF patient lung tissue and a fibrotic mouse model compared to controls. Importantly, treatment with an anti-fibrotic compound reduced DHLNL levels, indicating the assay’s potential utility for evaluating drug efficacy in fibrosis.
Elizabeth is currently a Scientist II at AbbVie within the High-Throughput, Automation, Bioassay, BioAnalysis (HT-ABBA) group, with a combined decade of hands-on experience in instrumentation and chromatography utilizing LC-MS/MS. She holds a master’s degree in pharmaceutical sciences with a concentration in forensic science from the University of Florida obtained in 2021. Working in a variety of industries such as food, toxicology, and pharmaceuticals has fostered her career and love of separation science.
“I now have a better understanding of how HPLC works and why column choice is so important.”
“It is a fantastic course for learning the theory of LC and method development”
“The instructor, Merlin Bicking, is very helpful. It was great to see how wiling he is to answer questions and all the variety of methods he worked on.”
“I am just beginning my position from out of school. I found the course very insightful in terms of preparation for my future career”
“It is very useful for me because having hands on experience, which helped me to understand more of HPLC.”
“Great class. Would easily take again and recommend Dr. Merlin anytime. Would love to take his other courses. If he had a book would quickly buy for reference.”
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Last Updated: January 9, 2026 by CCDG
Rapid LC-MS/MS evaluation of collagen and elastin crosslinks in human and mouse lung tissue
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Join us for a dinner and networking event Wednesday February 25, 2026 at the Westwood Tavern in Schaumburg, IL. The lecture will be presented by Elizabeth Sande. This presentation describes a rapid, efficient method for analyzing post-translational crosslinking in the extracellular matrix, which is crucial for understanding fibrotic diseases such as idiopathic pulmonary fibrosis (IPF). By streamlining LC-MS/MS sample analysis to just two minutes and introducing a tissue-based surrogate matrix for dihydroxylysinonorleucine (DHLNL), the process is less labor-intensive and provides more accurate quantification. Application of this method revealed elevated DHLNL levels in both IPF patient lung tissue and a fibrotic mouse model compared to controls. Importantly, treatment with an anti-fibrotic compound reduced DHLNL levels, indicating the assay’s potential utility for evaluating drug efficacy in fibrosis.
Elizabeth is currently a Scientist II at AbbVie within the High-Throughput, Automation, Bioassay, BioAnalysis (HT-ABBA) group, with a combined decade of hands-on experience in instrumentation and chromatography utilizing LC-MS/MS. She holds a master’s degree in pharmaceutical sciences with a concentration in forensic science from the University of Florida obtained in 2021. Working in a variety of industries such as food, toxicology, and pharmaceuticals has fostered her career and love of separation science.
REGISTER HERE
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LC School Reviews
“I now have a better understanding of how HPLC works and why column choice is so important.”
“It is a fantastic course for learning the theory of LC and method development”
“The instructor, Merlin Bicking, is very helpful. It was great to see how wiling he is to answer questions and all the variety of methods he worked on.”
“I am just beginning my position from out of school. I found the course very insightful in terms of preparation for my future career”
“It is very useful for me because having hands on experience, which helped me to understand more of HPLC.”
“Great class. Would easily take again and recommend Dr. Merlin anytime. Would love to take his other courses. If he had a book would quickly buy for reference.”