The Chicago Chromatography Group invites you to its Annual Open Board Meeting.

If you are interested in serving on the board, or just want to learn more about how CCDG works, we would love for you to join us on Thursday, March 3rd at 6 PM.

CLICK HERE TO REGISTER!

*Special Treat: All attendees will receive a Starbucks gift card and a chance for additional prizes.

Posters by: Edward G. Franklin, PhD, Technical Director of Chromatography Applications at Regis Technologies, Inc.

HPLC-UV Method Development for Baseline Resolution of 17 Cannabinoids

With ongoing discussions regarding the legalization of marijuana along with great interest in the potential medical benefits of hemp-derived products, there is increasing demand upon the cannabis testing industry for analytical determination of cannabinoid content. Current regulations concerning potency vary by jurisdiction, but usually require testing for the active forms of THC and CBD. In addition to those, California requires testing for the acid forms, THCA and CBDA, along with CBG and CBN. As regulations evolve, and as research interests in minor cannabinoids expand, it is important to have robust analytical methods in place that are capable of meeting needs. Herein, the baseline resolution of 17 cannabinoids by high performance liquid chromatography (HPLC) with UV detection is described. Chromatographic method development was performed with particular attention to speed of analysis and means of affecting selectivity for the improved resolution of critical pairs.

Click here to view poster.

Sample Loadability on Coated and Immobilized Polysaccharide-Based CSPs

Chiral stationary phases (CSPs) prepared by coating phenylcarbamate derivatives of amylose and cellulose on supporting silica gels have proven invaluable for the separation and purification of enantiomers in both high-performance liquid chromatography (HPLC) and supercritical fluid chromatography (SFC). One notable limitation of coated polysaccharide phases is the restricted use of certain organic mobile phases, such as acetone, chloroform, ethyl acetate, tetrahydrofuran (THF), and toluene. These solvents must be avoided as they can swell or dissolve the polysaccharide and destroy columns packed with coated CSPs. Immobilized versions of the same chiral selectors have expanded the capabilities of these phases to allow for the use of such solvents in analysis and sample purification. For some applications, however, practitioners have observed important differences in selectivity and sample loading capacities between the coated and immobilized versions. Herein, three examples of chiral separations performed using HPLC and SFC are discussed with respect to how these differences may be manifested and overcome or exploited to advantage.

Click here to view poster.

Edward G. Franklin, PhD, Technical Director of Chromatography Applications at Regis Technologies, Inc. Dr. Edward G. Franklin earned a B.S. in Biochemistry in 2006 and M.S. in Forensic Science in 2007 from Duquesne University in Pittsburgh, PA. In 2012, he received a PhD in Analytical Chemistry from The University of North Carolina at Chapel Hill under the direction of Dr. James Jorgenson. In over 10 years of HPLC-related experience, he has conducted research ranging from fundamental investigations of the kinetic performance of packed-bed HPLC columns, instrument and column development for ultra-high pressure separations of complex samples, and column stationary phase development for application-specific methods. In his current position as the Technical Director of Chromatography Applications at Regis Technologies, Inc., he develops new products and methods for chromatography solutions related to chiral and achiral separations using reversed-phase, normal phase, and supercritical fluid approaches.

CCDG proudly presents Merlin Bicking from ACCTA, Inc. as our speaker for our spring meeting that will take place on WebEx. Please click here for registration.

Your Autosampler Can Make Mixtures and Calibration Standards

Merlin K. L. Bicking, PhD, President and Senior Analytical Scientist, ACCTA, Inc.

We have automated or improved nearly every step in most chromatography methods, except for the preparation of calibration standards. High precision and accuracy methods still require Class A pipets and volumetric flasks, and large volumes of high purity solvents. This presentation will describe a simple procedure that uses a typical HPLC autosampler to prepare single or as multi-component mixtures of calibration standards at multiple levels. The method is most efficient when you have the ability to program the autosampler directly, but there are simple variations that can be developed without such programming. Multiple applications will be shown that demonstrate the ability to prepare standards that match manual preparations. This on-demand procedure has been successfully validated for GMP use and is an excellent alternative for high-cost standards.

Click here to view a poster preview of the presentation.

About the presenter:

Dr. Bicking has extensive analytical chemistry experience in academia, contract research, independent testing laboratories, consulting, and technical training. His professional history includes development of two EPA methods, as well as numerous methods in other regulated and non-regulated industries. His publications and presentations cover a wide range of topics, including liquid chromatography theory, derivatization, method optimization, and the use of experimental design strategies in analytical chemistry. He also develops and presents technical training seminars for analytical laboratory staff. He has been teaching the CCDG LC School for the last four years.

Pittcon, the world’s leading annual conference and exposition on laboratory science will be held March 1-5 at Chicago’s McCormick Place.

While there, stop by the CCDG booth and be sure not to miss the 2020 Separations Community Mixer, Tuesday evening, March 3, 5:30-9:30 pm, at Buddy Guy’s Legends.

Contact your chromatography supplier listed below to score a ticket to the hottest party at Pittcon.

The Chicago Chromatography Discussion Group has added a poster session at the beginning of each dinner meeting to provide an opportunity for chromatographers to present, demonstrate, and discuss their most recent achievements, practical experiences, novel ideas and challenges. The goal of the poster track is to encourage and facilitate the exchange within the chromatography community, foster collaboration, and, therewith strengthen the community as a whole.

At the end of the year, the governing board will award one poster $250 and a one year membership.

Submissions to the website should include either:

A draft of the actual poster including author(s) and affiliation(s); or

An extended abstract of the presented contribution comprising the following contents:

• Title
• Motivation and goals
• Background information if needed to understand the contribution

Deadline for posters is February 7th. Our next Networking, Dinner & Presentation Meeting is Tuesday February 11, 2020

Send your submission to chicagochromatography@gmail.com

CCDG is excited to announce the 2019 Gas Chromatography school.
September 24-26, 2019

About this Event

Co-sponsored by Roosevelt University and held on their campus, this course offers a well-designed combination of lecture and hands-on laboratory training. Students gain sufficient theoretical and practical background to perform independent work in gas chromatography.

This three day course, taught by Dr. Lee Polite from Axion, will provide instruction on a wide range of topics, including but not limited to:

Introduction to Gas Chromatography

GC Inlet systems

GC capillary columns and resolution

GC split ratio

Temperature programming and electronic pressure control

GC/MS, Headspace GC, Liquid GC

Method development and quantitative analysis

GC Troubleshooting and more!

This course is AACC accredited for 20 credit hours.

Registration fee: $995 (includes text, supplies and lunches) | $500 for students

Printed course material is provided, at no extra cost to the student, by the Chicago Chromatography Discussion Group.

Location: Roosevelt University
1400 N Roosevelt Blvd
Schaumburg, IL 60173

REGISTER HERE

Hello to all members and friends of CCDG. We have just wrapped up our 2018-2019 season, and throughout the year I have had the privilege of working with a talented and hard-working board to accomplish some amazing things.

This year, we changed our membership term and our sponsorship term to reflect the calendar year. This was done to help our members, as well as our board, to help keep track of renewals. We are also in the process of transitioning to a new program for membership renewal that will streamline the process of renewing membership

In the fall, we launched a brand-new website. We found this new website to be more user friendly, and enables us to keep our website updated in a timelier fashion. Our new website also allows us to share more pictures from our dinner lectures and our schools.

This year we were very excited to bring back our GC school, which was held at Roosevelt University in Schaumburg. It was a huge success with 13 students, and the instructor, Dr. Lee Polite went above and beyond for our students ensuring they left the class well informed.

Our GC school was such a success at Roosevelt University that we decided to move our LC school to the same location. Dr. Merlin Bicking once again led an outstanding course in which the students learned a great deal about liquid chromatography.

At our Young Scientist Dinner lecture, we awarded graduate student Sarah Lum with the Young Scientist Award. Her presentation on a novel way to quickly and efficiently analyze rape kits was both captivating and informative. We look forward to seeing what else Sarah will accomplish in her career.

We attended two conferences this year, the Midwest Association of Toxicology and Therapeutic Drug Monitoring in Cleveland, and the ACS Great Lakes Regional Meeting in Lisle. Attending conferences helps us to grow our organization and accomplish our motto of Sharing in Knowledge.

Lastly, we hosted four dinner lectures this year, ranging in topics from tips and tricks in gas chromatography to the challenges of teaching chromatography in a third world country. A huge thank you to all of our speakers this year, Eric Pavlich of Agilent, Gosia Medrecki, Kayla Watanabe, Sarah Lum, Dr. John Stuff of Gerstel, and Dr. Merlin Bicking of ACCTA.

Thank you all for supporting CCDG through membership, dinner lectures, or our schools, and I am looking forward to another wonderful year!

Regards,

Amy Alexander
CCDG President